Protein research is integral to targeted drug development as well as disease diagnosis. Aromatic amino acids in proteins such as tryptophan (Trp), tyrosine (Tyr) and phenylalanine (Phe) fluoresce when excited with UVC light. The shape and magnitude of the intrinsic fluorescence spectrum is used to identify local environment of amino acids, determine proximity relationships and measure events in living cells in real-time.
Time-resolved fluorescence provides more information about the molecular environment of the fluorophore than steady-state fluorescence measurements. Many macromolecular events occur on the same time scale as fluorescence decay. Thus, time-resolved fluorescence spectroscopy can be used to investigate these processes and gain insight into chemical surroundings of the fluorophore.
Benefits of Crystal IS LEDs for core protein research:
Narrow spectrum for selective excitation of different amino acids
Lack of forward heat and use of remote light sources eliminates thermal effects on samples
Instant on/off facilitates instantaneous modulation for time resolved measurements